Specific Histone Acetylation at the SOC1 locus in Arabidopsis thaliana
I will investigate the effects of specific histone acetylation on the locus of SOC1, a floral integrator gene. It is known that the acetylation status of chromatin modulates gene expression. I want to determine whether or not it is possible to modulate gene expression by specifically altering the histone acetylation status at the SOC1 locus using a fusion protein containing the DNA binding domain of OXS2, a transcription factor that specifically binds the SOC1 locus, and a histone acetyl transferase (HAT) in Arabidopsis thaliana. Following construction of a binary vector and introduction into plants, I will assess expression on a molecular and phenotypic basis, as upregulation of SOC1 will induce early flowering. If successful, this will enable locus-specific manipulation of transcription.
Message to Sponsor
- Major: Molecular and Cell Biology
- Mentor: David Ow, Plant and Microbial Biology