SURF

Roxanne Rajaii

Selection of Dosage Suppressors for the Lack of Nuclear Pik1

Cell responses are mediated by signal transduction pathways that involve protein and lipid kinase cascades. One of the two essential PtdIns 4-kinases in the budding yeast (S. cerevisiae), encoded by the PIK1 gene, localizes both to the Golgi and in the nucleus and has an essential function in both compartments. Pik1 in the Golgi functions in regulating secretion from the Golgi to the cell surface, but the processes that Pik1 helps regulate in the nucleus are not well understood. In an effort to understand the essential role of Pik1 in the nucleus, I have proposed a selection for dosage suppressors of the lethal phenotype of cells that lack nuclear Pik1 activity. Dosage suppressors are genes that, when over-expressed, are able to rescue the viability of cells that lack an otherwise essential gene function. Identifying the protein products of such dosage suppressors may allow me to discern what role PtdIns4P production, specifically by Pik1, plays in the nucleus and what processes this lipid regulates

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By becoming immersed in a multidisciplinary community such as SURF, we will each be able to further our knowledge and understanding of other disciplines, and in turn, achieve personal and intellectual growth in an environment free of financial worry. My interest in meeting others that are similarly motivated will allow me to learn how to better communicate with my peers and work as a tight-knit family, striving to reach a common goal, that of intellectual understanding and cooperation. Through this common interest and eagerness, we will undoubtedly grow as young individuals and gain a sense of confidence that will make it even easier for us to lead ourselves, and others, with a clear sense of direction.
  • Major: Molecular and Cell Biology
  • Mentor: Jeremy Thorner, Molecular and Cell Biology