SURF

Robert Potter

Understanding the Effects of AKT Pathway Inhibitors on the Pathogenesis of Mycobacterium Tuberculosis in Macrophages

Mycobacterium tuberculosis manipulates the host response to prevent bactericidal mechanisms and promote its own survival. AKT is one enzyme that has been linked to proliferation of M. tuberculosis within its target. As a serine/threonine protein kinase, AKT is an important enzyme in signal transduction pathways for apoptosis. Inhibition of AKT during infection leads to increase bacterial death. By looking at the downstream effects of the AKT pathway, our goal is to determine, why? This will be accomplished by using site directed mutagenesis to create multiple alterations to the structure of AKT in macrophages and then using inhibitors of AKT, stop the downstream effects at numerous junctures after being infected by a model organism, M. Smegmatis. Western blotting will be used to analyze the AKT signaling. We plan to infect the AKT mutant macrophages with fractionated M. tuberculosis and treat with inhibitors to compare to infection patterns of M. Smegmatis.

Message to Sponsor

The SURF Rose Hills experience has given me a chance to dive full time into scientific investigation with a peace of mind-knowing that the cost of living for the summer will be more than adequately financed. I would like to thank the Rose Hills sponsors for funding my personal needs as well as giving additional money to my lab so that I can apply my knowledge from Cal in a meaningful way. I would also like to thank my research mentors Dr. Sarah Stanley and Dr. Kim Sogi for the opportunity to work in such a fascinating field.
  • Major: Molecular and Cell Biology
  • Sponsor: Rose Hills Foundation
  • Mentor: Sarah Stanley, Public Health