Mutations affecting carotenoid biosynthesis in the photosynthetic model organism Chlamydomonas reinhardtii
I am characterizing a set of Chlamydomonas insertional mutants defective in carotenoid biosynthesis using pigment analysis, genetic crosses, and TAIL-PCR to connect mutations in specific genes with specific blocks in carotenoid biosynthesis,with the goal of elucidating in detail one or more steps in the Chlamydomonas carotenoid biosynthesis pathway. A better understanding of this is not only important for the understanding of the process of photosynthesis in general, but also in the comparison of carotenoid biosynthesis in the model organism Chlamydomonas with that in higher plants, possibly revealing interesting evolutionary and functional relationships between the genes involved in Chlamydomonas carotenoid biosynthesis and those involved in the carotenoid biosynthesis of other photosynthetic organisms.
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- Major: Molecular and Cell Biology, German
- Mentor: Krishna Niyogi, Plant and Microbial Biology