High-Throughput Imaging of DNA Holliday Junctions
DNA Holliday junctions (HJs) are double-stranded structures that promote genetic variation during DNA replication. Errors during separation of the two strands that comprise the HJ may lead to various biological issues contributing to human disease. The Redding Lab seeks to approach this problem by first learning more about the biophysical properties of HJs. We look to engineer a DNA curtain, a microfluidic assay capable of trapping HJs on a microscope slide for further analysis in real time observation. Once successful, we can see the effects of manipulating experiment design on HJs to learn more about the mechanism of its resolution.
Message to Sponsor
- Major: Bioengineering
- Mentor: Sy Redding