Dissection of Brainstem-Midbrain Circuits in Sensory Information Processing
The lateral parabrachial nucleus (LPB) is a region in the mammalian brain that is involved in regulating diverse behaviors associated with pain and aversion as well as feeding behaviors. Many neurons in the LPB are glutamatergic and project to widespread brain areas including the amygdala, periaqueductal gray, ventral tegmental area (VTA) and substantia nigra (SN). However, it is currently unknown whether subpopulations of LPB neurons, defined by their projection target, can be linked to specific behaviors. As an important step to gain knowledge of the functional heterogeneity of LPB cell populations, this project will utilize advanced anatomical methods to investigate projection-defined LPB neurons. In particular, I will focus on pathways from the LPB to the central amygdala (CeA) and VTA/SN dopamine regions in the ventral midbrain. My project involves (1) using anterograde and retrograde viruses to broadly characterize discrete LPB efferent pathways, (2) utilizing a brain clearing technique to identify whole brain projections, and (3) using immunohistochemical and in situ hybridization techniques to identify the neurochemical phenotype of projection-defined LPB neurons.
Message to Sponsor
- Major: MCB Neurobiology
- Sponsor: Pergo L&S
- Mentor: Stephan Lammel