Identifying Genes Involved in CRISPR/Cas9 Gene Editing

Summer 2017

Sharon Feng : Molecular and Cell Biology, Business Administration

Mentor: Chris Richardson

Genome editing is an emerging topic with immediate and significant implications in human health. Despite interest in therapeutic gene editing technologies, such as CRISPR/Cas9, interactions between genome editing reagents and human cells remain poorly understood. Prior work in the Corn Lab identified how human cells integrate single stranded DNA into Cas9 cut sites. My project will build upon this work to establish how cells respond to other gene editing techniques, namely the integration of double stranded DNA into genomic DNA.

I would like to thank the Rose Hills Foundation for allowing me to immerse myself in CRISPR/Cas9 genetics research this summer. Thanks to this fellowship, I have been able to complete a parallel project to a pooled screen that my mentor, Dr. Richardson, and I have just completed. I've had the opportunity to learn about new techniques and generate a new list of candidate genes that may play a role in CRISPR/Cas9 mediated DNA repair.